AFTPH (aftiphilin) is a component of clathrin-coated vesicles and the aftiphilin/p200/gamma-synergin complex involved in AP-1-mediated protein trafficking 1. Primary function includes regulating intracellular trafficking between the trans-Golgi network (TGN) and endosomes, including transferrin recycling and lysosomal enzyme transport 1. AFTPH localizes to early endosomes and the TGN 2 and is essential for acquiring regulated secretory phenotype in Weibel-Palade bodies 3. Mechanistically, AFTPH functions as a microRNA target. Neurotensin-induced miR-133α downregulates AFTPH to facilitate neurotensin receptor 1 (NTR1) recycling to the plasma membrane 2. Similarly, exosome-derived miR-199b-5p targets AFTPH, which interacts with NF-κB p65 to suppress inflammatory signaling 4. Disease relevance: AFTPH dysregulation is implicated in inflammatory bowel disease. The NTR1/miR-133α/AFTPH axis regulates colonic inflammation, with dysregulation observed specifically in ulcerative colitis patients 56. AFTPH shows elevated expression in multiple cancers (breast, lymphoma, lung, pancreatic) correlating with poor prognosis, suggesting oncogenic roles 7. AFTPH is also involved in Notch signaling during eye development 8.