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10 sources retrieved · Most recent: April 2026 · Index updated 15 days ago
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AGPAT4
1-acylglycerol-3-phosphate O-acyltransferase 4
Chromosome 6 · 6q26
NCBI Gene: 56895Ensembl: ENSG00000026652.16HGNC: HGNC:20885UniProt: Q9NRZ5
31PubMed Papers
20Diseases
0Drugs
0Pathogenic Variants
DATA QUALITY
✓ Experimental GO Evidence✓ Swiss-Prot Reviewed
protein binding1-acylglycerol-3-phosphate O-acyltransferase activityendoplasmic reticulumendomembrane systemcoronary artery diseaseangina pectorismetabolic syndromemyocardial infarction
✦AI Summary

AGPAT4 (1-acylglycerol-3-phosphate O-acyltransferase 4) is a mitochondrial outer membrane enzyme that catalyzes the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA) by incorporating acyl-CoA moieties, with particular specificity for polyunsaturated fatty acids like docosahexaenoic acid 1. The enzyme localizes to mitochondrial outer membranes and trans-Golgi network membranes, where it plays critical roles in membrane remodeling and phospholipid biosynthesis 12. In normal physiology, AGPAT4 regulates brain phospholipid composition, including phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol levels 2. However, AGPAT4 is aberrantly upregulated in multiple cancers and acts as an oncofetal protein promoting tumor progression. In colorectal cancer, elevated AGPAT4 supports tumor growth by suppressing LPA-mediated macrophage M1 polarization and T-cell activation 3. In hepatocellular carcinoma, AGPAT4 promotes tumor lineage plasticity and sorafenib resistance through mTOR/S6K signaling, and AGPAT4 inhibition synergizes with targeted therapy 4. AGPAT4 is also upregulated in endometriosis and thyroid carcinoma, correlating with poor prognosis 56. These findings establish AGPAT4 as a therapeutic target across multiple malignancies and non-neoplastic proliferative diseases.

Sources cited
1
AGPAT4/LPAATδ converts LPA to PA using acyl-CoA substrates; localizes to mitochondrial outer membranes and trans-Golgi network
PMID: 31428612
2
AGPAT4 is mitochondrial AGPAT/LPAAT that regulates brain phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol levels
PMID: 26417903
3
AGPAT4 aberrantly expressed in colorectal cancer; silencing suppresses CRC growth in xenografts and promotes M1 macrophage polarization via LPA
PMID: 32296017
4
AGPAT4 is oncofetal protein in hepatocellular carcinoma promoting tumor plasticity and sorafenib resistance via mTOR/S6K pathway; AGPAT4 inhibition sensitizes HCC to sorafenib
PMID: 40737432
5
AGPAT4 upregulated in endometriosis; reduced expression decreases endometrial stromal cell proliferation, invasion, and migration via Wnt3a/β-catenin signaling
PMID: 38850428
6
AGPAT4 identified as key lipid metabolism gene in thyroid carcinoma; high AGPAT4 expression correlates with worse prognosis
PMID: 40119647
7
AGPAT4 included in metabolism-related gene prognostic index for head and neck squamous cell carcinoma; associated with higher metabolic activity and worse outcomes
PMID: 35844514
Disease Associationsⓘ20
coronary artery diseaseOpen Targets
0.49Moderate
angina pectorisOpen Targets
0.47Moderate
metabolic syndromeOpen Targets
0.45Moderate
myocardial infarctionOpen Targets
0.42Moderate
Myocardial IschemiaOpen Targets
0.34Weak
hypertensionOpen Targets
0.34Weak
food allergyOpen Targets
0.33Weak
HypercholesterolemiaOpen Targets
0.33Weak
peripheral vascular diseaseOpen Targets
0.33Weak
nutritional deficiency diseaseOpen Targets
0.33Weak
systemic lupus erythematosusOpen Targets
0.33Weak
cardiovascular diseaseOpen Targets
0.32Weak
ovarian neoplasmOpen Targets
0.28Weak
metabolic diseaseOpen Targets
0.26Weak
bronchial diseaseOpen Targets
0.26Weak
bone neoplasmOpen Targets
0.16Weak
connective tissue neoplasmOpen Targets
0.16Weak
tuberculosisOpen Targets
0.16Weak
neoplasmOpen Targets
0.15Weak
Dupuytren ContractureOpen Targets
0.15Weak
Pathogenic Variants
No pathogenic variants reported on ClinVar for this gene.
View on ClinVar ↗
Related Genes
GPAT2Protein interaction98%PLPP2Protein interaction98%LPIN3Protein interaction96%PLPP5Protein interaction92%PLPP4Protein interaction92%MBOAT7Protein interaction92%
Tissue Expression6 tissues
Brain
100%
Bone Marrow
66%
Ovary
47%
Lung
29%
Heart
11%
Liver
5%
Gene Interaction Network
Click a node to explore
AGPAT4GPAT2PLPP2LPIN3PLPP5PLPP4MBOAT7
PROTEIN STRUCTURE
Preparing viewer…
AlphaFoldAI-predicted · UniProt Q9NRZ5
View on AlphaFold ↗
Constraintⓘ
LOEUFⓘ
0.46Moderately Constrained
pLIⓘ
1.00Intolerant
Observed/Expected LoF0.30 [0.20–0.46]
RankingsWhere AGPAT4 stands among ~20K protein-coding genes
  • #11,657of 20,598
    Most Researched31
  • #2,615of 17,882
    Most Constrained (LOEUF)0.46 · top quartile
Genes detectedAGPAT4
Sources retrieved10 papers
Response time—
📄 Sources
10▼
1
The Agpat4/LPA axis in colorectal cancer cells regulates antitumor responses via p38/p65 signaling in macrophages.
PMID: 32296017
Signal Transduct Target Ther · 2020
1.00
2
Unraveling the significance of AGPAT4 for the pathogenesis of endometriosis via a multi-omics approach.
PMID: 38850428
Hum Genet · 2024
0.90
3
AGPAT4 targeted covalent inhibitor potentiates targeted therapy to overcome cancer cell plasticity in hepatocellular carcinoma mouse models.
PMID: 40737432
Sci Transl Med · 2025
0.80
4
Mitochondrial acyltransferases and glycerophospholipid metabolism.
PMID: 27377347
Biochim Biophys Acta Mol Cell Biol Lipids · 2017
0.70
5
The Structure and Function of Acylglycerophosphate Acyltransferase 4/ Lysophosphatidic Acid Acyltransferase Delta (AGPAT4/LPAATδ).
PMID: 31428612
Front Cell Dev Biol · 2019
0.60