AK3 (adenylate kinase 3) is a mitochondrial enzyme that catalyzes nucleotide phosphoryl transfer reactions, specifically functioning as a GTP:AMP phosphotransferase and potentially using ITP as a phosphate donor 12. The enzyme is localized to the mitochondrial matrix and serves as a housekeeping gene expressed in all tissues except red blood cells 3. AK3 plays a crucial role in cellular energy metabolism by contributing to adenine nucleotide homeostasis and supporting high-energy phosphate transfer within mitochondrial compartments 3. The gene is mapped to chromosome 9, with multiple genomic loci including a processed pseudogene located within an intron of the NF1 gene on chromosome 9 4. Clinical relevance includes its involvement in 9p deletion syndromes, where AK3 is identified as one of 24 important genes affecting the majority of patients with this chr9 disorder 5. Additionally, AK3 demonstrates gene dosage effects in cases of 9p trisomy 6. In cancer research, AK3 has been identified as a target of miR-96-5p in breast cancer, where reduced AK3 expression correlates with decreased patient survival and increased cancer cell migration 7.