Based on limited published evidence, BBOF1 (basal body orientation factor 1) plays an essential role in sperm motility and male fertility by stabilizing the sperm flagellar axonemal structure 1. The protein is required for maintaining the integrity of the 9+2 axonemal microtubule arrangement and interacts with axonemal component MNS1 and outer dense fiber protein ODF2 to ensure their stability 1. BBOF1 is exclusively expressed in male germ cells from the pachytene stage onwards. Loss of BBOF1 results in reduced sperm motility due to missing microtubule doublets and male infertility, making it a candidate gene for asthenozoospermia 1. It is dispensable for motile cilia assembly and function.