CACHD1 (Cache Domain Containing 1) is a transmembrane protein that functions as a novel regulator of Wnt signaling pathways and neuronal development. CACHD1 can bind simultaneously to Lrp6 and Frizzled family Wnt co-receptors, modulating Wnt pathway activity 1. The protein plays a critical role in establishing neuronal asymmetry, as loss of CACHD1 function results in right-sided habenula neurons adopting left-sided identity in zebrafish 1. CACHD1 is expressed in neuronal progenitors and influences the timing of asymmetric neurogenesis patterns 1. Biallelic loss-of-function variants in CACHD1 cause a rare neurodevelopmental syndrome characterized by mild cognitive impairment, facial dysmorphism, and multisystem abnormalities 2. CACHD1 depletion in neural progenitors leads to reduced post-mitotic neuron percentages and enlarged neurospheres, with affected gene expression patterns related to Wnt signaling and neurodevelopment 2. Additionally, CACHD1 serves as a biomarker in hepatocellular carcinoma progression, where knockdown inhibits cancer cell survival and proliferation 3. Recent studies have also identified CACHD1 as an alternative initiator of Wnt signaling 4, highlighting its importance in cellular signaling networks beyond its originally proposed role in calcium channel regulation.