CAMSAP3 is a key microtubule-organizing protein that specifically binds and stabilizes the minus-ends of non-centrosomal microtubules. The protein forms dimers through its C-terminal α-helix domain, which enhances microtubule-binding affinity and enables dynamic regulation of microtubule minus-ends 1. CAMSAP3 plays a critical role in establishing epithelial cell polarity by tethering non-centrosomal microtubules to the apical cortex, thereby orienting the apical-to-basal polarity of microtubule arrays in epithelial cells 2. In motile cilia, CAMSAP3 facilitates proper basal body orientation and formation of central microtubule pairs, which are essential for synchronized ciliary beating 3. The protein's dysfunction is associated with primary ciliary dyskinesia-like phenotypes, including defective ciliary morphology and impaired directional flow generation 3. In cancer biology, CAMSAP3 acts as a tumor suppressor in lung cancer, where its depletion promotes cellular senescence through ERK1/2 inactivation 4, enhances invasion and angiogenesis via HIF-1α mRNA stabilization 5, and contributes to osimertinib resistance by disrupting EGFR trafficking 6. Low CAMSAP3 expression correlates with poor prognosis in lung adenocarcinoma patients 57.