CDC25C is a dual-specificity protein phosphatase that serves as a critical regulator of cell cycle progression, particularly the G2/M transition 1. The protein functions by directly dephosphorylating CDK1, thereby activating CDK1/cyclin B complexes required for mitotic entry 1. CDC25C exists in distinct phospho-isoforms during mitosis, with different subcellular localizations and functions: the pT67 form associates with condensed chr5 throughout mitosis, while the pT130 form localizes to centrosomes and interacts with Plk1 2. The protein forms a tetrameric complex with a native molecular mass of 220 kDa and demonstrates phosphatase activity against artificial substrates 1. CDC25C expression and function are tightly regulated through multiple mechanisms, including transcriptional control and post-transcriptional modifications such as N6-methyladenosine methylation 3. The protein is subject to proteasomal degradation mediated by TRIB2, which promotes its ubiquitination 4. Dysregulation of CDC25C has significant clinical implications, as elevated expression correlates with poor prognosis in non-small cell lung cancer 53, making it a potential therapeutic target for cancer treatment.