CENPI (centromere protein I) is a core centromeric protein essential for chromosome X and kinetochore assembly. As a component of the CENPA-CAD complex, CENPI facilitates CENPA incorporation into centromeres and is required for proper localization of checkpoint proteins MAD1L1 and MAD2 to kinetochores, ensuring accurate mitotic progression 1. Beyond its canonical centromeric function, CENPI has emerged as a significant oncogene across multiple cancer types. It is consistently overexpressed in lung adenocarcinoma, colorectal cancer, breast cancer, glioblastoma, and hepatocellular carcinoma 12345. In these malignancies, CENPI promotes tumorigenesis through distinct molecular mechanisms: Wnt/β-catenin signaling in breast cancer 3, RAS/MEK/ERK pathway activation in hepatocellular carcinoma 5, and arginine/proline metabolism regulation in glioblastoma 4. CENPI knockdown suppresses cell proliferation, migration, invasion, and epithelial-mesenchymal transition across cancer models 21. High CENPI expression correlates with poor prognosis and treatment resistance, positioning it as a potential biomarker and therapeutic target 13. Clinically, CENPI mutations have been linked to steroid-sensitive nephrotic syndrome via X-linked inheritance 6, and anti-CENPI autoantibodies appear in scleroderma patients, particularly those with concurrent autoimmune liver disease 7.