CEP57 is a coiled-coil scaffold protein at the pericentriolar matrix (PCM) that serves as a critical regulator of centrosome organization and centriole duplication 1. The protein functions through multivalent interactions, undergoing liquid-liquid phase separation to catalyze microtubule nucleation and aster formation via its leucine-zipper-containing C-terminal domain 12. CEP57 forms a complex with Cep63 and Cep152 during interphase, with its N-terminal motifs binding Cep63 to facilitate proper PCM assembly and Plk4 recruitment for procentriole initiation 34. Beyond centrosome function, CEP57 localizes to kinetochores where it binds the KMN network component Mis12 and recruits Mad1-Mad2 complexes, playing essential roles in spindle checkpoint activation 5. CEP57 and its homolog CEP57L1 maintain centriole engagement during interphase, preventing premature disengagement that would trigger re-duplication and aneuploidy 6. Loss-of-function CEP57 mutations cause mosaic variegated aneuploidy syndrome 2, characterized by PCM disorganization, centriole disengagement, and chromosome 11 errors 17. Notably, CEP57 overexpression in prostate cancer paradoxically correlates with improved prognosis, likely due to mitotic dysfunction limiting cell proliferation 8.