CKAP2L (cytoskeleton associated protein 2-like) is a microtubule-associated protein that plays a critical role in mitotic spindle formation and cell cycle regulation. The protein exhibits cell cycle-dependent expression, peaking during G2/M phase, and localizes to the mitotic spindle apparatus during mitosis and to the nucleus during interphase 1. CKAP2L demonstrates microtubule-stabilizing properties and its overexpression leads to microtubule bundling and prolonged mitosis 1. The protein promotes cell cycle progression by facilitating the transition from G2 to M phase and is essential for proper chromosome 2 in neural progenitor cells 2. Loss-of-function mutations in CKAP2L cause Filippi syndrome, a rare autosomal recessive disorder characterized by microcephaly, growth failure, syndactyly, and intellectual disability 3. Patients with Filippi syndrome show defective spindle pole localization of CKAP2L, resulting in disorganized spindle microtubules and chromosome 2 defects 3. Beyond developmental disorders, CKAP2L is overexpressed in multiple cancers where it promotes proliferation, invasion, and metastasis through activation of signaling pathways like AKT/mTOR 4. The protein serves as a potential biomarker for cancer prognosis and immunotherapy response 2.