DDX47 is a DEAD-box RNA helicase with multifaceted roles in RNA metabolism and genome stability. Functionally, DDX47 is required for efficient ribosome biogenesis through its involvement in pre-rRNA processing 1, with proper nucleolar localization dependent on the nucleolar protein NOP132 1. Beyond ribosome assembly, DDX47 functions as an R-loop resolvase that protects cells from harmful R-loop accumulation, which can cause genome instability 2. The helicase works in concert with other factors like senataxin and DDX39B to resolve nucleolar R-loops 3, and interacts with FANCD2 to suppress R-loops during mild replication stress 4. DDX47 is involved in apoptotic pathways and RNA splicing processes 1. Clinically, DDX47 dysregulation is associated with multiple malignancies. High DDX47 expression in lung adenocarcinoma correlates with poor prognosis and promotes cell proliferation and migration 5. DDX47 was identified as a prognostic RNA binding protein in renal cell carcinoma 6 and shows diagnostic value in sepsis/septic shock, where it associates with immune cell regulation and apoptotic pathways 7. These findings suggest DDX47 dysfunction contributes to carcinogenesis and immune dysregulation through altered R-loop homeostasis and RNA metabolism.