DHX35 is a DEAH-box RNA helicase that functions in both pre-mRNA splicing and antiviral innate immunity. In the spliceosome, DHX35 partners with GPATCH1 to maintain catalytic dormancy and promote splicing fidelity by suppressing cryptic splice sites and enabling accurate splicing of introns with divergent spacing 1. The protein also functions as a cytosolic nucleic acid sensor that recognizes double-stranded RNA and DNA, translocating from the nucleus to the cytoplasm upon immune stimulation 2. There, DHX35 interacts with RIG-I and the adaptor protein MAVS to amplify antiviral signaling, enhancing production of interferon-β, IL-6, and CXCL10 in airway epithelial cells 2. Beyond splicing and immunity, DHX35 is implicated in craniofacial development, with variants associated with nose shape variation 3, and has been identified in cancer-related transcriptomic analyses including prostate cancer bicalutamide resistance 4 and breast carcinogenesis pathways 5. Recent evidence indicates DHX35 mRNA is upregulated in ischaemic cardiomyopathy 6, suggesting involvement in cardiac disease pathogenesis. The gene thus represents a multifunctional regulator bridging RNA processing, immune defense, and disease-related cellular dysfunction.