DIXDC1 (DIX domain containing 1) is a positive regulator of canonical Wnt signaling that functions downstream of Wnt and upstream of Axin 1. As a DIX domain-containing protein, DIXDC1 activates WNT3A signaling and regulates JNK activation through interactions with DVL2 and AXIN1. The long isoform (l-DIXDC1) uniquely contains an actin-binding domain and localizes to filamentous actin at focal adhesions, suggesting dual functions in both β-catenin signaling and cytoskeletal regulation 2. DIXDC1 drives cell proliferation through PI3K/AKT pathway activation, promoting G1/S phase transition via upregulation of cyclin D1 and downregulation of p21 1. Clinically, DIXDC1 is significantly upregulated in multiple cancers including glioma, pancreatic, gastric, and prostate cancers, where high expression correlates with poor prognosis, advanced tumor grade, and increased metastatic potential 3456. In gastric cancer, DIXDC1 promotes invasion and metastasis through Wnt pathway activation, increasing matrix metalloproteinases and decreasing E-cadherin 5. Notably, DIXDC1 expression is downregulated in hepatocellular carcinoma, serving as an independent prognostic factor 7. During neural development, DIXDC1 promotes neuronal differentiation while inhibiting gliogenesis 8, establishing its broader developmental roles beyond oncogenic pathways.