DPPA2 (Developmental Pluripotency Associated 2) is a chr3-binding transcription factor that serves as a key epigenetic priming factor regulating cell fate transitions and maintaining developmental competency 1. The protein functions by binding to target gene promoters and maintaining the active epigenetic status of developmental genes through regulation of bivalent chr3 domains containing both H3K4me3 and H3K27me3 modifications 12. DPPA2 works in partnership with DPPA4 as a heterodimer to facilitate chr3 remodeling during somatic cell reprogramming to induced pluripotency, achieving reprogramming efficiencies exceeding 80% when co-expressed with standard pluripotency factors 3. The protein directly regulates zygotic genome activation by upregulating DUX expression, with its activity negatively controlled by SUMO E3 ligase PIAS4 through sumoylation 4. DPPA2 promotes cell proliferation and pluripotency maintenance through activation of the PI3K/AKT/GSK3β/β-catenin signaling pathway and subsequent LEF1 upregulation 5. Clinically, DPPA2 functions as a cancer/testis antigen, being normally expressed in germ cells and trophoblasts but aberrantly reactivated in various malignancies 6. Its expression pattern during human preimplantation development suggests distinct roles in late-stage embryonic reprogramming processes, particularly in blastocysts and primordial germ cells 7.