DPPA5 (developmental pluripotency associated 5) is a cytoplasmic mRNA-binding protein that plays a crucial role in maintaining embryonic stem cell pluripotency and supporting cellular reprogramming. Mechanistically, DPPA5 enhances pluripotency by directly interacting with and stabilizing NANOG protein through post-transcriptional mechanisms 1, rather than affecting NANOG transcription. DPPA5 is specifically and highly expressed in pluripotent stem cells including human primordial germ cells, embryonic germ cells, and embryonic stem cells, with expression rapidly downregulated during differentiation 2. Epigenetically, DPPA5 promoter methylation is associated with stem cell differentiation and aberrant silencing in tumor cells; DNA methyltransferase inhibitors can reactivate DPPA5 expression 3. Clinically, DPPA5 serves as a reliable pluripotency marker 2 and has been identified as a hub gene associated with asthma severity in bioinformatics analyses 4. Additionally, DPPA5 expression increases in embryos exposed to stem cell-derived bioactive materials, correlating with enhanced developmental potential 5. These findings establish DPPA5 as an important regulator of pluripotency maintenance and a valuable biomarker for stem cell biology and disease pathology.