DYNLT2B (also known as TCTEX1D2) is a dynein light chain that serves as a non-catalytic accessory component of the cytoplasmic dynein-2 complex, a motor protein essential for intraflagellar transport (IFT) 1. It interacts with intermediate chain WDR60 and other dynein subunits to form a functional retrograde IFT machinery 2. DYNLT2B localizes to the ciliary base and is required for proper ciliogenesis and retrograde ciliary protein trafficking 13. In spermatozoa, DYNLT2B functions as an intra-acrosomal protein contributing to cargo movement 4. Loss of DYNLT2B function impairs retrograde IFT, destabilizing the retrograde IFT dynein motor 3. Mutations in TCTEX1D2/DYNLT2B cause Jeune asphyxiating thoracic dystrophy (short-rib thoracic dysplasia 17), a ciliopathy characterized by skeletal dysplasia, respiratory insufficiency, and potentially situs inversus and cardiac defects 35. The condition exhibits variable expressivity, with some patients presenting dental anomalies including molar-incisor malformation and tooth agenesis 56. DYNLT2B plays an auxiliary role in dynein-2 assembly, with WDR60 showing more critical contributions to retrograde trafficking 2.