KIF27 is a kinesin-4 family motor protein that plays an essential role in motile ciliogenesis. It functions as a microtubule-associated scaffold protein at the ciliary transition zone, a diffusion barrier at the base of motile cilia 1. Rather than relying on its motor activity, KIF27 maintains transition zone architecture and enables proper incorporation of motility-generating proteins into the axoneme 1. KIF27 is a mammalian ortholog of the Drosophila Hedgehog pathway regulator Costal-2, sharing a conserved domain structure that includes a kinesin motor domain and binding domains for signaling proteins 2. Loss of KIF27 results in profound axonemal structural defects and disrupted cilia beating, leading to organismal phenotypes that recapitulate primary ciliary dyskinesia (PCD) 1. The gene was identified as a candidate tumor-suppressor gene within a commonly deleted region in acute myeloid leukemia (AML) samples with del(9q), suggesting potential haploinsufficiency in leukemogenesis 3. KIF27 is also implicated in Hedgehog signaling networks relevant to gastric and pancreatic cancer development 4, though its primary characterized role is in ciliary structural integrity rather than signaling regulation in mammals 5.