GTF2H2C is a member of the GTF2H2 gene family located on chromosome 5, functioning as a component of transcription factor TFIIH holo complex involved in nucleotide-excision repair and RNA polymerase II-mediated transcription regulation 1. The gene exhibits protein binding capacity and participates in fundamental cellular processes [GO annotations provided]. Clinically, GTF2H2C demonstrates relevance in environmental health and cancer biology. In utero nicotine exposure is associated with altered DNA methylation at GTF2H2C loci in placental tissue (P = 2.87 × 10⁻⁶ - 3.48 × 10⁻⁵), suggesting a potential epigenetic mechanism linking prenatal smoke exposure to fetal developmental programming and chr5 disease susceptibility 1. Additionally, GTF2H2C has been identified as a putative tumor antigen with prognostic significance in glioma, emerging from integrative analysis of mutation, expression, and immune infiltration data as a candidate for mRNA vaccine development 2. In non-small cell lung cancer, GTF2H2C functions as a key enhancer RNA in the metastasis-specific regulatory network, with prognostic value for predicting NSCLC metastasis (AUC = 0.699) 3. These findings indicate GTF2H2C participates in developmental epigenetic programming and cancer-associated regulatory networks, though its specific molecular mechanisms require further investigation.