HOOK2 is a microtubule-binding protein that serves multiple critical functions in cellular organization and transport. As a component of the FTS/Hook/FHIP complex, HOOK2 promotes vesicle trafficking and fusion via the HOPS complex, contributing to endosome organization and transport 1. The protein localizes to the centrosome throughout all cell cycle phases and directly binds to centriolin/CEP110, playing essential roles in establishing and maintaining centrosomal structure and function 1. HOOK2 is crucial for microtubule organization, as its disruption results in loss of radial microtubule organization and defects in microtubule regrowth 1. The protein contributes to aggresome formation by promoting microtubule-based delivery of protein aggregates to pericentrosomal locations 2. HOOK2 also regulates primary cilium morphogenesis by interacting with and stabilizing PCM1, which recruits Rab8a for membrane transport to the cilium 3. Additionally, HOOK2 interacts with the polarity protein PAR6α to control centrosome orientation during polarized cell migration and Golgi reorientation 4. Disease relevance includes differential DNA methylation patterns in HOOK2 associated with type 2 diabetes and obesity, showing sex-specific methylation differences 56, and potential involvement in ovarian cancer through MYC promoter regulation 7.