KIF1C is a kinesin-3 motor protein that mediates bidirectional microtubule-based cargo transport, functioning as both an anterograde and retrograde transporter 1. As a plus-end-directed motor, KIF1C transports diverse cargoes including dense core vesicles, organelles, and mRNAs to neuronal and cellular protrusions 12. Transport activation requires relief of KIF1C autoinhibition, achieved through binding of adaptor proteins PTPN21 and Hook3 to its stalk region, which release inhibitory interactions between the motor domain and stalk 13. KIF1C participates in mRNA localization by directly interacting with cargo through RNA-binding adaptors: APC recognizes specific 3'UTR sequences, while CNBP binds GA-rich motifs and recruits KIF1C to target mRNAs 24. Notably, KIF1C exhibits a non-canonical function in promoting dynein-driven retrograde lysosomal transport via Hook3 interaction, independent of its motor activity 56. Biallelic loss-of-function mutations in KIF1C cause Hereditary Spastic Paraplegia type SPG58 (Spastic Ataxia 2), a neurodegenerative motoneuron disease 7. Additionally, KIF1C variations have been implicated in cardiac myxoma pathogenesis through transcriptional regulation of PRKAR1A 8. These findings establish KIF1C as a multifunctional motor protein critical for neuronal function and cellular trafficking.