KRT32 (keratin 32) is a structural protein predominantly localized in basal keratinocytes that plays critical roles in both skin immune homeostasis and hair follicle integrity. In the skin epidermis, KRT32 functions as an anti-inflammatory regulator by binding to NEMO and promoting its K48-linked polyubiquitination and degradation, thereby inhibiting NF-κB pathway activation 1. Loss-of-function mutations in KRT32 result in NF-κB hyperactivation and are significantly associated with pityriasis rubra pilaris (PRP), an inflammatory papulosquamous dermatosis, with Krt32 knockout mice exhibiting PRP-like dermatitis 1. In hair follicles, KRT32 is expressed in both the inner root sheath and hair fiber cuticle, contributing to intermediate filament organization and structural integrity 2. A heterozygous variant (p.Thr99Ile) in KRT32 disrupts binding to KRT82, compromising intermediate filament structure and causing loose anagen hair syndrome characterized by weak anagen hair anchorage 3. Additionally, KRT32 expression is significantly reduced in alopecia areata lesions compared to normal scalp, correlating with loss of hair follicle structural integrity 4. KRT32 also marks differentiation-specific inner root sheath differentiation in basal cell carcinomas, where its expression correlates with reduced proliferative index 5. These findings establish KRT32 as a multifunctional keratin essential for maintaining skin immune homeostasis and hair follicle architecture.