LHFPL4 (LHFPL tetraspan subfamily member 4) is a transmembrane protein that regulates inhibitory synapse formation and function. It acts as a traffic controller at the synapse by dictating neuroligin preference and enabling dynamic reassignment between excitatory and inhibitory postsynaptic domains 1. LHFPL4 maintains gamma-aminobutyric acid type A receptors (GABAARs) clustering and stabilizes their associated scaffold proteins at inhibitory synaptic sites, functioning in concert with neuroligin-2 (NLGN2) to recruit or stabilize GABAARs 2. This regulatory mechanism is critical for the molecular plasticity of inhibitory synapses, where LHFPL4 contributes to the dynamic equilibrium of synaptic binding sites and postsynaptic density organization 3. Beyond neurotransmission, LHFPL4 methylation patterns have been identified as epigenetic age-related markers in forensic applications, showing significant association with chr3 age in blood and buccal cell samples 45. Additionally, aberrant CpG island methylation of LHFPL4 has been identified as a novel methylation target in cervical cancer, occurring in 55% of cases and potentially relevant for early detection and risk prediction 6. These findings establish LHFPL4 as a multifunctional protein critical for synaptic inhibitory balance and as a biomarker for age estimation and cancer detection.