LIMK2 is a serine/threonine kinase that plays essential roles in actin cytoskeleton dynamics and cell cycle regulation. Its primary function involves phosphorylating cofilin to regulate actin filament polymerization, acting downstream of RhoA/ROCK signaling pathways 12. LIMK2 also regulates microtubule organization through phosphorylation of TPPP and controls mitotic spindle orientation 3. The kinase exhibits dual tumor-regulatory roles: it can promote cancer progression by facilitating centrosome clustering through MST4-mediated NPM1 phosphorylation, enabling survival of cancer cells with supernumerary centrosomes 3. Conversely, LIMK2 can act as a tumor suppressor in colorectal cancer, where its downregulation leads to β-catenin nuclear translocation and enhanced metastasis 4. LIMK2 expression is dysregulated across multiple cancer types and shows promise as both a therapeutic target and biomarker 56. In smooth muscle, LIMK2 promotes contraction and proliferation, making it a potential target for treating overactive bladder 1. The kinase's pleiotropic functions extend beyond actin regulation to include roles in ciliogenesis suppression and various signaling pathways 7.