MAN2A2 encodes an alpha-mannosidase enzyme localized to the Golgi apparatus that catalyzes a critical step in N-glycan maturation 1. The enzyme controls the conversion of high mannose to complex N-glycans by trimming mannose residues, representing the final hydrolytic step in the N-glycan processing pathway 1. MAN2A2 functions as a central hub in multi-enzyme assemblies within Golgi membranes, forming distinct molecular complexes with other N-glycan processing enzymes including MGAT1, MGAT2, MGAT3, and MGAT4B to facilitate efficient complex N-glycan synthesis 2. The gene is highly expressed in brain regions, and pathogenic variants cause autosomal recessive congenital disorders of glycosylation (CDG) characterized by neurological involvement, autism spectrum disorder, cognitive delay, and facial dysmorphism 13. Functionally defective MAN2A2 results in accumulation of immature hybrid-type N-glycans and decreased complex N-glycans 13. Beyond developmental roles, MAN2A2 expression is upregulated in pathological conditions including cholangiocarcinoma progression under high glucose-ROS conditions 4 and shows altered expression in cardiovascular disease contexts 5. The enzyme's dysfunction in neuroinflammatory conditions is evidenced by downregulated MAN2A2 in LPS-activated microglia 6.