MEAK7 (mammalian Enhancer-of-Akt-1-7) is a lysosomal protein that regulates mTOR signaling and V-ATPase activity. As a positive activator of alternative mTOR signaling 1, MEAK7 recruits mTOR to the lysosome through direct binding to V-ATPase subunits A, B, D, and E 23, facilitating mTOR complex assembly necessary for nutrient sensing. This interaction occurs through MEAK7's conserved TLDc domain and C-terminal region 2. In cancer contexts, MEAK7 promotes cell proliferation and migration via enhanced phosphorylation of ribosomal protein S6 and 4E-BP1 12. Notably, MEAK7 uniquely activates rather than inhibits V-ATPase among TLDc-containing proteins 4. The upstream regulation of MEAK7 occurs through microRNA-1911-3p, which targets MEAK7 mRNA and suppresses mTOR signaling in lung cancer cells 1. Additionally, MEAK7 participates in alternative mTOR complexes downstream of DDR1-mediated collagen signaling in pancreatic cancer fibrosis 5, linking collagen sensing to metabolic regulation. MEAK7 expression increases in response to immune modulatory signals in microglia 67, suggesting roles in neuroinflammation and cell morphology regulation.