MYO16 (myosin XVI) is a vertebrate-specific, actin-based motor protein predominantly expressed in neural tissues during critical developmental periods 1. The protein exists as two principal isoforms: Myo16a (tailless, cytoplasmic) and Myo16b (full-length, with cytoplasmic and nuclear localization) 1. Both isoforms contain an N-terminal ankyrin repeat domain that binds protein phosphatase catalytic subunits 1. Functionally, MYO16 serves as a signal transduction element integrating cell signaling to actin cytoskeleton reorganization 2. In the cytoplasm, Myo16b activates the PI3K pathway and recruits the WAVE complex to regulate neuronal morphogenesis and actin cytoskeleton reorganization 1. Nuclear Myo16b may regulate cell cycle progression 1. MYO16 interacts with neuronal proteins including MAP1B and KIRREL3, contributing to neurodevelopment 3. Dysregulation of MYO16 expression is implicated in multiple neuropsychiatric disorders including autism, schizophrenia, and bipolar disorder 1, and variants in MYO16 associate with structural brain connectivity measures 4. Beyond neurodevelopment, MYO16-related long non-coding RNAs regulate glucose metabolism and invasion in lung and bladder cancers 5, 6.