Nestin (NES) is a type VI intermediate filament protein essential for neural development and stem cell function. During brain and eye development, NES promotes disassembly of phosphorylated vimentin intermediate filaments during mitosis, facilitating proper distribution of cytoskeletal components to daughter cells 1. NES is widely utilized as a marker for neural stem/progenitor cells (NSCs/NPCs) in developing and adult nervous systems 1. NES cells derived from induced pluripotent stem cells serve as robust in vitro models for studying human neuronal differentiation, ciliogenesis, and neurodevelopmental processes 23. During neuronal differentiation, NES expression dynamics correlate with developmental stages and ciliary gene enrichment, supporting its role in neural progenitor proliferation and maturation 3. Beyond neurodevelopment, NES is implicated in multiple malignancies. Recent studies demonstrate elevated NES expression in BRAF-mutant metastatic melanoma, where it co-expresses with epithelial-mesenchymal transition (EMT) markers (SNAI1, LOXL3), suggesting involvement in melanoma progression and metastatic potential 4. NES is also associated with glioblastoma, colorectal carcinoma, and other cancers, likely reflecting aberrant stem cell-like properties in transformed cells. The clinical significance of NES extends from developing neural progenitor identification for regenerative medicine applications to understanding cancer stem cell biology and potential therapeutic targeting of EMT pathways.