NHSL2 (NHS like 2) is an X-linked gene belonging to a protein family that includes NHS and NHSL1, characterized by functional WAVE homology domains 1. As a member of this family, NHSL2 is predicted to function in protein binding and cell differentiation processes. NHSL2 has been identified as a component of competing endogenous RNA (ceRNA) networks involved in Alzheimer's disease pathology, where it was dysregulated in the hippocampus of 5×FAD mice alongside alterations in circRNA-miRNA interactions affecting neuronal development and synaptic function 2. Additionally, NHSL2 expression is significantly altered in bone marrow stem cells (BMSCs) from adolescent idiopathic scoliosis (AIS) patients with osteopenia, where reduced NHSL2 levels correlate with impaired autophagy activity and abnormal BMSC differentiation, leading to decreased osteogenic capacity and increased adipogenic differentiation 3. These findings suggest NHSL2 plays roles in regulating cell differentiation and potentially autophagy-dependent processes. However, the specific molecular mechanisms by which NHSL2 functions in these contexts remain to be elucidated, and direct functional studies of NHSL2 itself are lacking in the provided literature.