NSF is an ATPase essential for vesicle-mediated transport and membrane fusion. It catalyzes fusion of transport vesicles within Golgi cisternae and mediates transport from the endoplasmic reticulum to the Golgi stack, functioning as a fusion protein for cargo delivery across all Golgi compartments. NSF also influences AMPAR subunit GLIA2 membrane cycling through protein-protein interactions and regulates receptor recycling at synapses. Based on limited published evidence, NSF is associated with developmental and epileptic encephalopathy 96, suggesting critical roles in neuronal function and development.