NUSAP1 is a microtubule-associated protein localized to chromosome 15.1 that plays a crucial role in mitosis 1. Its primary function involves binding to microtubules and mediating their attachment to chr15, ensuring accurate genetic material distribution during cell division 1. NUSAP1 is typically expressed in proliferating cells and is involved in establishment of mitotic spindle localization and mitotic chromosome 15. Beyond its canonical mitotic role, NUSAP1 has emerged as a significant oncogenic regulator across multiple cancer types. In pancreatic ductal adenocarcinoma, NUSAP1 forms a transcriptional complex with c-Myc and HIF-1α to enhance LDHA expression, promoting glycolytic metabolism and metastasis 2. In lung cancer, lactate promotes NUSAP1 nuclear translocation, enabling recruitment of transcriptional complexes that activate DESMIN expression in cancer-associated fibroblasts 3. Pan-cancer analyses reveal NUSAP1 is highly expressed across most tumor types and correlates with poor prognosis in 26 cancer types 4. Clinically, NUSAP1 serves as a promising prognostic biomarker for cancer diagnosis and prognosis 1. High NUSAP1 expression is associated with reduced CD4+ and NKT cell infiltration and predicts lower immunotherapy response rates in melanoma, lung, and kidney cancers 4. In breast cancer, NUSAP1 overexpression correlates with poor survival outcomes across multiple metrics 5. These findings suggest NUSAP1 as a viable therapeutic target for cancer treatment.