PEX14 is a peroxisomal membrane protein that functions as a critical component of the PEX13-PEX14 docking complex, serving as the initial docking site for the PEX5 receptor during peroxisomal protein import 1. The docking complex forms a large import pore (approximately 9 nm diameter) through which PEX5-bound cargo proteins are translocated into the peroxisome matrix 2. Mechanistically, PEX14 interacts with PEX5 and PEX13 through its N-terminal domain, while its C-terminal domain undergoes phase separation to create condensates that recruit receptor-cargo complexes 3. Beyond import, PEX14 directly binds tubulin and facilitates peroxisome movement along microtubules 4. During de novo peroxisome biogenesis, PEX14 targets mitochondria where it is selectively released into vesicular pre-peroxisomal structures that subsequently mature upon fusion with endoplasmic reticulum-derived vesicles 4. PEX14 also serves as a molecular docking site for optineurin on the peroxisomal membrane, enabling recruitment of autophagic machinery for selective peroxisome degradation (pexophagy) 5. Additionally, Ser232-phosphorylation of PEX14 suppresses catalase import, representing a cellular defense mechanism against oxidative stress 6. Mutations in PEX14 cause peroxisome biogenesis disorder complementation group K, characterized by defective peroxisomal protein import and loss of peroxisome integrity 1.