PEX26 is a peroxisomal membrane docking factor that anchors the PEX1-PEX6 AAA-ATPase complex to peroxisomal membranes 123. This docking function is essential for the PEX1-PEX6 complex to mediate extraction and recycling of the PEX5 cargo receptor from the peroxisomal membrane, a critical step in peroxisomal matrix protein import 123. PEX26 is a tail-anchored protein with a C-terminal transmembrane domain and interacts with PEX6 through its N-terminal region 4. Alternative splicing produces a cytosolic isoform (PEX26Δex5) lacking the transmembrane domain, which can still support peroxisome biogenesis, suggesting functional flexibility 45. During de novo peroxisome biogenesis, PEX26 is transiently recruited to mitochondria-derived pre-peroxisomal structures before maturation via endoplasmic reticulum-derived vesicles 6. Mutations in PEX26 cause peroxisome biogenesis disorders (PBD), including severe Zellweger spectrum disorder (ZSD), with approximately 90% of ZSD cases harboring mutations in PEX26 or related genes 7. Beyond peroxisomal function, PEX26 acts as a metastasis suppressor in colorectal cancer, with low expression correlating with poor survival and promoting epithelial-mesenchymal transition through Wnt pathway activation 8. In cancer drug resistance, PEX26 silencing enhances pexophagy (peroxisomal autophagy) and sensitizes cells to therapy, with low PEX26 expression associated with improved survival in lymphoma, lung cancer, and melanoma 9.