PRPF6 (pre-mRNA processing factor 6) is a core component of the U4/U6-U5 tri-snRNP complex within the spliceosome, functioning as an essential regulator of pre-mRNA splicing 1. Beyond its canonical splicing role, PRPF6 localizes to the connecting cilium and is critical for primary cilium biogenesis 2. The protein also enhances androgen receptor and glucocorticoid receptor transactivation, indicating roles in nuclear hormone signaling. Germline mutations in PRPF6 cause autosomal dominant retinitis pigmentosa (RP60), a progressive inherited retinal disease characterized by vision loss and night blindness 3. The tissue-restricted phenotype despite PRPF6's ubiquitous expression remains mechanistically unclear 4. Additionally, PRPF6 mutations associate with periventricular heterotopia, where defective PRPF6 causes missplicing of microtubule-associated kinases essential for proper brain development 5. Beyond germline disease, PRPF6 is upregulated in cancer contexts. In ovarian cancer, PRPF6 promotes metastasis and paclitaxel resistance through alternative splicing regulation of SNHG16 lncRNA 6. In hepatocellular carcinoma, USP13-mediated stabilization of PRPF6 enhances proliferation via AKT-mTOR pathway activation 7. These findings reveal PRPF6 as a multifunctional protein relevant to splicing, ciliogenesis, and cancer biology.