RAB25 is an epithelial-cell-specific small GTPase that regulates intracellular membrane trafficking and endocytic recycling 1. As a member of the Rab GTPase family, RAB25 cycles between inactive GDP-bound and active GTP-bound states to recruit effectors controlling vesicle formation, movement, and fusion 1. The protein promotes invasive cell migration by localizing integrin α-V/β-1 at pseudopodial tips and regulates epithelial morphogenesis through claudin-4 localization at tight junctions 1. RAB25 interacts with myosin 5B to regulate transcytosis and functions in both fast and slow endocytic recycling pathways alongside Rab4 and Rab11 family members 2. RAB25 plays a complex, context-dependent role in cancer. It functions as an oncogene in ovarian cancer, where overexpression promotes aggressiveness and associates with drug resistance through selective clonal expansion 3. Conversely, RAB25 acts as a tumor suppressor in colon cancer, where decreased expression promotes neoplastic development 4. This dichotomy reflects RAB25's role in regulating growth factor and adhesion molecule recycling, with implications for proliferation and invasion 5. Recently, RAB25 was identified as activating noncanonical endoplasmic reticulum stress pathways in alcohol-associated liver disease through interaction with GCN1, promoting hepatic lipid metabolism dysregulation and inflammation 6. Additionally, RAB25 expression is downregulated in hypospadias foreskin tissue, suggesting developmental roles in urethral morphogenesis 7.