RHOV (ras homolog family member V) is an atypical Rho GTPase that functions as a key regulator of cytoskeletal dynamics and cellular behavior through activation of the JNK/c-Jun signaling pathway 1. Unlike classical Rho family members, RHOV possesses N- and C-terminal extension regions, undergoes unusual GDP/GTP cycling, and is modified by palmitoylation rather than prenylation 2. The protein contains a proline-rich motif that enables interaction with SH3 domain-containing adaptor proteins like GRB2 and NCK2, with activity further regulated by PKA-mediated phosphorylation at Ser25 3. RHOV plays critical roles in cancer progression, promoting proliferation, migration, and invasion in lung adenocarcinoma through JNK/c-Jun pathway activation and regulation of epithelial-to-mesenchymal transition markers 1. The protein is essential for transcoelomic metastasis in ovarian cancer, where it functions as a detachment-responsive GTPase that enhances anoikis resistance and multicellular aggregate formation 4. RHOV expression is dysregulated across multiple cancer types, serving as either an oncogene or tumor suppressor depending on the specific cancer context 5. Clinically, RHOV overexpression correlates with poor prognosis in several cancers including lung adenocarcinoma 6 and represents a potential therapeutic target and prognostic biomarker 7.