RIPOR1 (RHO family interacting cell polarization regulator 1), also known as FAM65A, is a key effector protein in directional cell migration and polarization. As a downstream effector of Rho GTPases (RHOA, RHOB, and RHOC), RIPOR1 functions as an adapter protein linking active Rho proteins to STK24 and STK26 kinases 1. This interaction promotes Golgi reorientation toward the leading edge during polarized cell migration, a process dependent on the PDCD10/CCM3 pathway 1. RIPOR1 undergoes phosphorylation by upstream kinases upon chemokine stimulation, which alters its subcellular localization and affects Rho protein interactions, ultimately modulating cell polarity and migration 2. Notably, while UniProt describes RIPOR1 as positively regulating Golgi reorientation, recent evidence indicates RIPORs can also exert suppressive effects on RHO activity 2. Clinically, RIPOR1 expression associates with poor prognosis across multiple malignancies, particularly colorectal and lung squamous cell carcinoma, where elevated levels correlate with enhanced cell proliferation, invasion, and migration 34. These findings suggest RIPOR1 as a potential prognostic biomarker and therapeutic target in cancer.