STK25 is a serine/threonine kinase that functions as a critical regulator of cellular metabolic homeostasis and stress responses. As part of STRIPAK complexes, STK25 participates in multiple signaling pathways including Hippo, MAPK, and inflammatory signaling 1. The kinase localizes to the Golgi apparatus where it regulates protein transport, cell polarity, and migration 2. Mechanistically, STK25 responds to oxidative stress and metabolic signals to control lipid storage and glucose homeostasis 2. During alcohol-associated liver disease, STK25 inhibits PKA activity through phosphorylation, suppressing fatty acid oxidation and promoting hepatic steatosis 3. In immune cells, STK25 phosphorylates IRF5 at Thr265, promoting pro-inflammatory cytokine production downstream of TLR signaling 4. STK25 also regulates the Hippo pathway by phosphorylating LATS1/2, controlling YAP activation 5. Clinically, STK25 elevation associates with multiple diseases. High STK25 expression correlates with atherosclerosis progression 6, colorectal cancer development 7, and systemic lupus erythematosus 4. STK25 knockout enhances anti-PD-1 immunotherapy efficacy by increasing PD-L1 stability 7, positioning STK25 inhibition as a therapeutic strategy for metabolic disorders, atherosclerosis, autoimmune diseases, and cancer immunotherapy resistance.