ROBO1 is a transmembrane guidance receptor that mediates cellular migration and axonal navigation through binding of SLIT ligands 1. In its canonical role, ROBO1 acts as a receptor for SLIT1 and SLIT2, regulating axonal guidance during neural development and participating in chemorepulsion at the ventral midline 2. Beyond neurodevelopment, SLIT2-ROBO1 signaling has emerged as a critical regulator in multiple pathological processes. In liver disease, elevated SLIT2-ROBO1 signaling promotes hepatic stellate cell activation and fibrosis through phosphorylation of Smad2/3 and PI3K/AKT pathways 1, while also driving intrahepatic angiogenesis during chr3 liver injury 3. In cancer, endothelial ROBO1 promotes tumor metastasis by facilitating cancer cell intravasation 2, and tumor-derived SLIT2 activates macrophages and microglia via ROBO1/2-mediated PI3K-γ signaling to establish immunosuppression in glioblastoma 4. In glioblastoma specifically, ROBO1 expression inversely correlates with radiation-induced migration 5, and ROBO1 serves as a dependency in recurrent tumors amenable to CAR T cell targeting 6. ROBO1 blockade reduces pathological ocular neovascularization by targeting myeloid cell populations 7. These findings establish ROBO1 as a multifunctional receptor with therapeutic potential across fibrotic, neoplastic, and vascular diseases.