TOM1 (target of myb1 membrane trafficking protein) functions as an adapter protein crucial for intracellular membrane trafficking of ubiquitinated proteins, participating in autophagy, receptor recycling, and immune signaling pathways 1. The protein binds polyubiquitinated proteins via its GAT domain and forms complexes with TOLLIP to recruit ubiquitin-conjugated proteins onto early endosomes 1. TOM1 acts as a myosin VI adapter protein, targeting MYO6 to endocytic structures and facilitating autophagosomal delivery of endocytic cargo and autophagosome-lysosome fusion 2. Through interaction with autophagy receptors like TAX1BP1, CALCOCO2/NDP52, and OPTN, TOM1 plays essential roles in selective autophagy processes 34. The protein undergoes desuccinylation by SIRT5, which stabilizes TOM1 and promotes autophagy in myocardial ischemia-reperfusion injury models 5. TOM1 also functions as a negative regulator of IL-1β receptor signaling, with reduced levels observed in Alzheimer's disease brain tissue 6. A pathogenic G307D variant causes severe early-onset autoimmunity and immunodeficiency by disrupting TOM1-TOLLIP interactions, impairing autophagosome-lysosome fusion, and causing excessive inflammatory pathway activation 7.