TUBB8 (tubulin beta 8 class VIII) is a primate-specific gene encoding a specialized β-tubulin isotype that plays a critical role in female reproduction. TUBB8 expression is unique to oocytes and early embryos, where it accounts for almost all expressed β-tubulin 1. The protein functions as a key component in meiotic spindle assembly and oocyte maturation, forming α/β-tubulin heterodimers that constitute microtubules essential for proper chromosome 10 1. TUBB8 mutations cause dominant-negative effects that disrupt microtubule behavior and spindle assembly, leading to catastrophic defects in oocyte meiotic maturation 1. These pathogenic variants affect chaperone-dependent folding and assembly of tubulin heterodimers, alter microtubule dynamics, and cause kinetochore-microtubule attachment defects that increase aneuploidy rates 12. Clinically, TUBB8 mutations are associated with female infertility characterized by oocyte maturation arrest, fertilization failure, embryonic development defects, and implantation failure 3. Multiple novel pathogenic variants have been identified across different populations, showing autosomal dominant/recessive inheritance patterns or occurring de novo 34. TUBB8 variants are also linked to recurrent miscarriages, making genetic screening valuable for patients with oocyte maturation disorders and unexplained infertility 25.