VAMP1 (vesicle-associated membrane protein 1) is a SNARE complex component that mediates synaptic vesicle fusion and neurotransmitter release at the presynaptic membrane 1. The protein functions by forming complexes with other presynaptic proteins to facilitate the targeting and fusion of transport vesicles to their target membranes, enabling GABA and other neurotransmitter release 2. VAMP1 mutations cause congenital myasthenic syndrome-25 (CMS-25), an autosomal recessive neuromuscular disorder characterized by impaired neuromuscular signal transmission 3. Clinical features of VAMP1-related CMS-25 include muscle weakness, ptosis, feeding difficulties, and motor developmental delay, with emerging evidence of cerebellar involvement and nephrolithiasis in some cases 4. VAMP1 mutations also cause hereditary spastic paraplegia through impaired SNARE complex formation and synaptic vesicle fusion dynamics 5. Additionally, VAMP1 genetic variants associate with Behçet's disease-related uveitis susceptibility 6, and reduced VAMP1 expression in prefrontal cortical interneurons may contribute to schizophrenia pathophysiology by impairing GABAergic inhibition and cortical gamma oscillations 2. Pharmacological interventions including pyridostigmine and salbutamol show variable efficacy in CMS-25 patients 4.