VTI1A encodes a vesicle-associated membrane protein (v-SNARE) that plays crucial roles in intracellular membrane trafficking and neuronal function. The protein mediates retrograde transport from endosomes to the trans-Golgi network (TGN) by forming SNARE complexes with syntaxin 6, syntaxin 16, and VAMP-4 1. VTI1A is predominantly localized to the Golgi apparatus and TGN, where it facilitates membrane fusion events critical for vesicle trafficking 1. In neurons, VTI1A has specialized functions including synaptic vesicle recycling, dense core vesicle secretion, and support of cortical development 2. Loss of VTI1A/VTI1B function in mice causes severe cortical malformations, including depletion of neural progenitor pools and disorganization of cortical layer 5, leading to defective corticospinal projections 3. The protein also maintains proper Golgi organization, as VTI1A/VTI1B-deficient neurons show altered distribution of Golgi markers and disrupted cargo sorting 4. Clinically, VTI1A genetic variants are associated with increased cancer risk, particularly for colorectal, breast, lung cancers and glioma 56. Additionally, VTI1A DNA methylation levels correlate with physical activity levels, suggesting potential epigenetic regulation 7.