VAMP3 is a SNARE protein that mediates vesicle fusion during intracellular membrane trafficking. Its primary function involves facilitating retrograde transport from late endosomes to the trans-Golgi network and regulating vesicle-mediated secretion 1. Mechanistically, VAMP3 functions as a v-SNARE that interacts with t-SNAREs (such as syntaxin 4 and SNAP23) to enable membrane fusion events critical for protein and microRNA secretion 2. VAMP3 participates in diverse cellular processes including autophagy-dependent and -independent secretion pathways, where it mediates fusion of cargo-containing vesicles with the plasma membrane 34. Disease relevance is substantial across multiple pathologies. VAMP3 genetic variants are associated with periodontitis susceptibility, sharing loci with atherosclerotic cardiovascular disease, suggesting common inflammatory pathways 5. In neuroblastoma, reduced VAMP3 expression correlates with worse outcomes, and VAMP3 suppression promotes cancer cell proliferation and inhibits apoptosis 1. VAMP3 is implicated in immune evasion within triple-negative breast cancer through CD73 membrane translocation 6. Additionally, VAMP3 participates in wound healing through autophagy regulation and facilitates bacterial meningitis pathogenesis by mediating transferrin receptor transcytosis across the blood-brain barrier 78. Clinically, VAMP3 represents a potential therapeutic target for cancer treatment and infectious disease prevention.