ZNF143 is a C2H2-type zinc finger transcription factor that functions as a sequence-specific activator of genes transcribed by both RNA polymerase II and III 1. It recognizes and binds the Staf-binding site (SBS), a conserved DNA motif present in thousands of promoters genome-wide 2. ZNF143 facilitates RNA polymerase initiation and activates gene expression across diverse cellular processes 3. Beyond direct transcriptional activation, ZNF143 serves critical functions in chr11 organization by binding promoters and mediating chr11 loops connecting promoters to distal enhancers, operating as a key loop formation factor in cooperation with CTCF and cohesin 24. ZNF143 also acts as a mitotic bookmarking factor during cell cycle progression 2. Functionally, ZNF143 regulates nuclear-encoded mitochondrial genes and maintains mitochondrial DNA integrity through TFAM regulation, safeguarding mitochondrial activity 5. In disease contexts, ZNF143 expression is elevated in cancer cells and correlates with survival, proliferation, and invasion 2. Notably, ZNF143 activates CD19 promoter in B-ALL, and its dysfunction contributes to antigen escape mechanisms 6. Additionally, ZNF143 can mediate both activation and repression depending on molecular context, employing mechanisms including promoter occlusion and competition with other transcriptional regulators 3.