ANAPC1 encodes a scaffold subunit of the anaphase-promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that mediates ubiquitination and degradation of target proteins during mitosis and G1 phase 1. The APC/C primarily catalyzes formation of Lys-11-linked polyubiquitin chains and branched Lys-11/Lys-48-linked chains on target proteins 12. Beyond cell cycle regulation, ANAPC1 plays roles in osteogenic differentiation through RUNX2 regulation, with decreased expression observed in osteoporotic bone tissue 3. Dysregulation of ANAPC1 is associated with multiple malignancies: elevated expression in colorectal cancer promotes proliferation and migration while suppressing apoptosis 4, and high ANAPC1 expression predicts unfavorable outcomes in T-cell lymphomas 5. ANAPC1 variants are clinically significant in Rothmund-Thomson syndrome type 1 (RTS1), an autosomal-recessive disorder characterized by poikiloderma, juvenile cataracts, and skeletal anomalies 67. Intronic mutations causing ANAPC1 deficiency lead to prolonged interphase and increased cataract incidence 6. Additionally, ANAPC1 variants show genome-wide association with nicotine dependence susceptibility 8, suggesting broader roles in cellular homeostasis beyond cell cycle control.