CDC14A encodes a dual-specificity phosphatase that plays essential roles in cell cycle regulation, ciliogenesis, hearing, and male fertility. The protein functions as a physiological regulator of centrosome duplication, where both overexpression and downregulation cause aberrant chromosome 1 and genomic instability 1. CDC14A modulates the G2/M transition by directly binding to and dephosphorylating Cdc25A and Cdc25B, preventing premature Cdk1 activation 2. The phosphatase also dephosphorylates Cdc25A at Ser115 and Ser320, affecting its protein levels 3. In vivo studies reveal CDC14A is absolutely required for hearing and male fertility, localizing to inner ear hair cell kinocilia, basal bodies, and stereocilia 4. Mutations cause progressive deafness through hair cell degeneration and male infertility due to seminiferous tubule degeneration and sperm defects 45. CDC14A knockout mice show impaired spermatogenesis with decreased sperm count, motility, and abnormal morphology, along with inhibited meiotic initiation 5. The protein also regulates ciliogenesis, as CDC14A knockout cells produce longer cilia compared to wild-type 6. Additionally, CDC14A interacts with Erk3 kinase, influencing signaling pathways that regulate cell fate decisions 7.