CENPW (centromere protein W) is a centromeric protein essential for kinetochore assembly and chromosome 6. As a component of the CENP-T-W-S-X heterotetrameric complex, CENPW binds and supercoils DNA, playing a fundamental role in kinetochore assembly and mitotic progression [UniProt annotation]. The protein is part of the CENPA-NAC complex involved in recruiting CENPA-CAD complexes and incorporating newly synthesized CENPA into centromeres. Clinically, CENPW overexpression is associated with poor prognosis in hepatocellular carcinoma (HCC), where elevated CENPW levels correlate with unfavorable outcomes 1. CENPW knockdown in HCC cells inhibits proliferation, migration, and invasion while inducing G2/M phase arrest and apoptosis, potentially through E2F signaling inactivation 2. Similarly, in clear cell renal cell carcinoma (ccRCC), elevated CENPW expression associates with reduced overall survival and is recognized as an independent negative prognostic factor 3. CENPW silencing in KIRC inhibits cell proliferation, migration, and invasion while increasing PD-L1 and E-cadherin expression, implicating roles in immune responses and epithelial-mesenchymal transition 4. CENPW was identified as a hub biomarker in HCC diagnostic prediction models 5 and as one of eight immune evasion-related genes prognostic for immunotherapy response in ccRCC 6. Additionally, CENPW variants associate with liver volume and skeletal muscle phenotypes, with sex-specific metabolic effects 7.