CKMT1A (creatine kinase, mitochondrial 1A) is a mitochondrial enzyme that catalyzes the reversible phosphoryl transfer between ATP and creatine phosphate, playing a central role in energy homeostasis in tissues with high energy demands 1. The protein localizes to the mitochondrial inner membrane and functions as part of the cellular creatine kinase circuit for ATP buffering 1. Mechanistically, CKMT1A participates in maintaining cellular energetics through the phosphocreatine biosynthetic pathway 1. It is highly expressed in brown adipose tissue alongside uncoupling protein 1, suggesting involvement in both coupled and uncoupled energy expenditure 2. Unlike CKB, CKMT1A silencing does not directly affect F1F0 ATP synthase sensitivity, indicating distinct regulatory roles among creatine kinase isoforms 3. CKMT1A exhibits context-dependent roles in cancer biology. In breast cancer, CKMT1A is highly expressed in primary tumors promoting viability, but downregulated in metastatic lesions where it contributes to invasive phenotype through ROS-mediated mechanisms 4. In endometrial cancer, elevated CKMT1A expression associates with advanced FIGO stages and poor overall survival, suggesting prognostic significance 5. Similarly, in hepatocellular carcinoma, CKMT1A upregulation via the n335586/miR-924 regulatory axis promotes cell migration and invasion 6. Pan-cancer analysis confirms CKMT1A overexpression across most tumor types, with associations to glycolytic and metabolic pathway activation 7.