CKMT2 (creatine kinase, mitochondrial 2) is a mitochondrial enzyme that reversibly catalyzes phosphate transfer between ATP and creatine phosphate, playing a crucial role in cellular energy metabolism 1. The enzyme is localized to the mitochondrial inner membrane and is essential for energy transduction in metabolically active tissues including skeletal muscle, heart, and brain. Beyond its traditional role in creatine phosphorylation, CKMT2 has emerged as a critical regulator of mitochondrial homeostasis independent of insulin action 1. In skeletal muscle, reduced CKMT2 expression is associated with impaired mitochondrial respiration, membrane potential, and glucose oxidation, contributing to metabolic dysfunction in type 2 diabetes 1. Conversely, CKMT2 overexpression enhances mitochondrial function and is upregulated by exercise training 1. In cancer biology, CKMT2 expression patterns vary across tumor types, with potential prognostic value and correlations with immune infiltration 2. The gene also gives rise to an antisense long non-coding RNA (CKMT2-AS1) that regulates various cancer-related pathways, including gastric cancer progression through STAT3 signaling and hepatocellular carcinoma development via miRNA sponging mechanisms 34.