DRC3 (dynein regulatory complex subunit 3, also known as LRRC48) is a structural component of the nexin-dynein regulatory complex (N-DRC), a critical regulatory complex located in the axoneme of motile cilia and sperm flagella 1. DRC3 functions to maintain alignment and integrity of the distal axoneme and regulate microtubule sliding in motile axonemes 2. Structurally, DRC3 localizes to the L1 projection of the nexin linker within the N-DRC, where it directly interacts with dynein motors and other N-DRC subunits including DRC1, 2, 4, 5, 7, and 8 2. Loss of DRC3 function causes asthenozoospermia and male infertility; a homozygous frameshift variant (c.644dup) resulting in premature translational arrest eliminates DRC3 protein detection in sperm, destroys flagellar ultrastructure, and severely reduces sperm motility 1. The variant also disrupts DRC3 interactions with other N-DRC components 1. Clinically, DRC3 mutations represent a genetic cause of male infertility affecting approximately 6% of infertile males with oligoasthenoteratozoospermia 3. DRC3 defects result in abnormal sperm tail beating and reduced ATP production 3, making DRC3 mutations important for clinical genetic diagnosis of male infertility.